Biohydrogen production from sugarcane bagasse by integrating dark- and photo-fermentation

Biohydrogen production from sugarcane bagasse by integrating dark- and photo-fermentation

Biohydrogenproductionfromsugarcanebagassebyintegratingdark-and

Biohydrogen production from sugarcane bagasse by integrating dark- and photo-fermentation

photo-fermentation

PankajK.Rai,S.P.Singh ,R.K.Asthana,ShwetaSingh

CentreofAdvancedStudyinBotany,BanarasHinduUniversity,Varanasi221005,India

highlights

AcidandenzymepretreatedSCBwasutilizedtoproduceH2. Effectiveremovaloffermentationinhibitorsbyadsorbentresin.

SSFFandtwo-stepfermentationwaseffectivelyutilizedforH2production.

articleinfoabstract

Hydrogenproductionfromsugarcanebagasse(SCB)byintegratingdark-fermentationbyEnterobacteraerogenesMTCC2822andphoto-fermentationbyRhodopseudomonasBHU01wasinvestigated.TheSCBwashydrolysedbysulphuricacidandthehydrolysatedetoxi edbypassingthroughadsorbentresincolumn(AmberliteXAD-4)toremovetheinhibitoryfurfural,andsubjectedtodark-fermentation.ThecellulosicresiduefromacidhydrolysiswashydrolysedbythenewisolateCellulomonas mitoreleasesugarsforH2productionbyE.aerogenes,throughsimultaneoussacchari cation, ltrationandfermenta-tion(SSFF).CumulativeH2productionduringdark-fermentationandSSFFwas1000and613ml/L,respectively.Thespentmediaofdark-fermentationandSSFFwereutilizedforphoto-fermentationbyRhodopseudomonasBHU01.ThecumulativeH2productionwas755ml/Lfordark-fermentationand351ml/LforSSFFspentmedium.

Ó2013ElsevierLtd.Allrightsreserved.

Articlehistory:

Received16September2013

Receivedinrevisedform19October2013Accepted23October2013

Availableonline12November2013Keywords:

SugarcanebagasseAcidhydrolysisSSFF

H2Production

1.Introduction

Hydrogen,apromisingalternativetofossilfuelsandacleanen-ergycarrier(DasandVeziroglu,2001),isproducedbiologicallyviabiophotolysis,dark-fermentationandphoto-fermentation.Sugarssuchasglucose,fructose,galactose,arabinose,lactoseandsucrosearethewidelystudiedsubstratesforbiohydrogenproduction(KumarandDas,2000;LinandJo,2003;Raietal.,2012).Asthecostofthesubstrateisofprimeconcernfortheeconomicsofbio-hydrogenproduction,thereisneedtogoforcheaperandabundantfeedstocksformakingtheprocesscost-effective.Theuseoforganicwastesfromindustriesandagriculturenotonlysupportsgreenenergygenerationbutalsohelpsinbioremediation(VenkataMohan,2009).Alargefractionofwastesfrommunicipal,industrialandagriculturalsectorcomprisinglignocellulosicbiomass,isrenew-ableandinexpensive,andthuswellsuitedforbiofuelproduction.Oneofthemajorlignocellulosicmaterialfoundinlargequantities

Correspondingauthor.Tel.:+910542230714647(O)/2414560(R);fax:+91

05422366402.

E-mailaddress:spsinghbhu@http://www.mianfeiwendang.com(S.P.Singh).

Biohydrogen production from sugarcane bagasse by integrating dark- and photo-fermentation

0960-8524/$-seefrontmatterÓ2013ElsevierLtd.Allrightsreserved.http://www.mianfeiwendang.com/10.1016/j.biortech.2013.10.117

especiallyinatropicalcountrylikeIndia,issugarcanebagasse(SCB)producedduringcanemilling(Singhetal.,2007).

Thebagasseiscomposedofcellulose(40–45%),hemicellulose(30–35%),lignin(20–30%).Ingeneral,1metrictonofsugarcanegenerates280kgofbagasse,about50%ofthisresidueisusedinsu-garmills/distilleriesastheenergysourcewhiletheremainingiscompostedorusedinpaperindustry(Pengetal.,2009).Celluloseinbagasseisahighlyorderedmoleculeconsistingoflinearinsolu-blepolymersofrepeatingb-1,4linkedb-Dglucopyranoseunits.Themainproblemintheuseofcelluloseisitscrystallinestructurethatmakesitdif culttohydrolyze.ThisnecessitatesuseofnoveltechniquestoachieveoptimumhydrolysisandtheextractionoffermentablesugarsfromtheSCBcellulose.Hydrogenproductionfromlignocellulosicfeedstockinvolvespre-treatmentbyacid/alka-liorbyothermeanstoopenupitscrystallinestructure,followedbyenzymatichydrolysisofcelluloseandhemicellulose(s)toyieldsugarsthatcanbeutilizedforfermentativeH2production(Panagiotopoulosetal.,2013a).Forenzymatichydrolysisofthelignocellulosicmaterial,moststudiesusedtheexpensivecommer-cialenzymeswhichaddtothecostoffermentationandthuslimitcommercializationofcellulose-basedhydrogenproduction(Storketal.,2009).Theenzymatichydrolysisandfermentationcanbe

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