质粒图谱pGEX

pGEX-1λT (27-4805-01)

Thrombin

↓Stop codonsEcoR IBamH I

pGEX-2T (27-4801-01)

Thrombin

↓Stop codonsBamH ISma IEcoR I

pGEX-2TK (27-4587-01)

Thrombin

Kinase

↓Stop codonsBamH ISma IEcoR I

pGEX-4T-1 (27-4580-01)

Thrombin

↓Stop codonsSal IXho INot IBamH I

pGEX-4T-2 (27-4581-01)

Thrombin

↓Leu Val Pro Arg Gly SerPro Gly Ile Pro Gly Ser Thr Arg Ala Ala Ala Ser

BamH IEcoR ISma ISal INot I

pGEX-4T-3 (27-4583-01)

Thrombin

↓Stop codonsEcoR ISma INot I

pGEX-3X (27-4803-01)

Factor Xa

Stop codonsBamH ISma IEcoR I

pGEX-5X-1 (27-4584-01)

Factor Xa

Stop codonsBamH IEcoR ISma ISal IXho INot I

pGEX-5X-2 (27-4585-01)

Factor Xa

Stop codonBamH INot IEcoR ISal IXho I

pGEX-5X-3 (27-4586-01)

Factor Xa

Stop codonsSma ISal INot I

pGEX-6P-1 (27-4597-01)

PreScission Protease

↓BamH IEcoR ISma ISal IXho INot I

pGEX-6P-2 (27-4598-01)

PreScission Protease

↓Leu Glu Val Leu Phe Not IBamH IEcoR ISal IXho I

pGEX-6P-3 (27-4599-01)

PreScission Protease

↓EcoR ISma IXho INot IBamH I

Bal Iut

PtacBspM I

gl

at

h

ase

nsfer-traeSnio

Aat II

pAm

pSj10 Bam7Stop7

r

Pst I

pGEX

~4900 bp

Nar IEcoR V

BssH II

Mlu I

pBR322ori

acI

p4.5

AlwN I

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